ITCH Rabbit Polyclonal Antibody

CAT#: TA326703

Rabbit Polyclonal ITCH Antibody

Product Images

Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH
• Formulation: ITCH antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: ITCH antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 99 kDa; Observed: 99 kDa
• Gene Name: itchy E3 ubiquitin protein ligase
• Database Link:
  NP_113671
  Entrez Gene 16396 MouseEntrez Gene 311567 RatEntrez Gene 83737 Human
  UniProt ID Q96J02
• Background: The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1). HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2). In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3). Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4).
• Synonyms: ADMFD; AIF4; AIP4; dJ468O1.1; NAPP1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis