KAP1 (TRIM28) Goat Polyclonal Antibody

CAT#: TA326731

Goat Anti-KAP1 Antibody

Product Images

TA326731 (1ug/ml) staining HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:2000; WB: 1-3µg/ml
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-KQFNKLTEDKADVQ, from the internal region of the protein sequence according to NP_005753.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tripartite motif containing 28
• Database Link:
  NP_005753
  Entrez Gene 21849 MouseEntrez Gene 116698 RatEntrez Gene 403689 DogEntrez Gene 10155 Human
  UniProt ID Q13263
• Synonyms: KAP1; PPP1R157; RNF96; TF1B; TIF1B
• Note: Approx 100kDa band observed in nuclear lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 88.5kDa according to NP_005753.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3µg/ml.

Reference Data

• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors