Cytokeratin 18 (KRT18) Goat Polyclonal Antibody

CAT#: TA326739

Goat Anti-keratin 18 Antibody

Product Images

TA326739 (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:32000; WB: 0.1-0.3µg/ml
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EELDKYWSQQIE, from the internal region of the protein sequence according to NP_000215.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: keratin 18
• Database Link:
  NP_000215
  Entrez Gene 16668 MouseEntrez Gene 294853 RatEntrez Gene 477601 DogEntrez Gene 3875 Human
  UniProt ID P05783
• Synonyms: CYK18; K18
• Note: Approx 48kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kDa according to NP_000215.1). Recommended concentration: 0.1-0.3µg/ml. Reported variants represent identical protein: NP_000215.1, NP_954657.1.

Reference Data

• Protein Pathways: Pathogenic Escherichia coli infection