Ubiquitin (UBB) Rabbit Polyclonal Antibody

CAT#: TA326763

Ubiquitin B Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Ubiquitin B antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000-1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human Ubiquitin B
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin B
• Database Link:
  NP_061828
  Entrez Gene 22187 MouseEntrez Gene 192255 RatEntrez Gene 7314 Human
  UniProt ID P0CG47
• Background: Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the e-amino group of the target protein lysine residue. Combinatorial interactions of different E2 and E3 proteins result in substrate specificity. UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa.
• Synonyms: HEL-S-50

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease