Bak (BAK1) Rabbit Polyclonal Antibody

CAT#: TA326768

Rabbit anti-BAK1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of MCF7 cell lines, using BAK1 antibody.

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  Immunohistochemistry of paraffin-embedded human kidney cancer using Bak1 antibody at dilution of 1:200 (x400 lens).

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  Immunohistochemistry of paraffin-embedded human stomach cancer using Bak1 antibody at dilution of 1:200 (x400 lens).

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  Immunohistochemistry of paraffin-embedded rat heart using BAK1 antibody at dilution of 1:100 (x400 lens).

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  Immunofluorescence analysis of HeLa cell using Bak1 antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;ICC 1:50 - 1:200;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: N term -peptide of human BAK1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23 kDa
• Gene Name: BCL2 antagonist/killer 1
• Database Link:
  NP_001179
  Entrez Gene 12018 MouseEntrez Gene 116502 RatEntrez Gene 578 Human
  UniProt ID Q16611
• Background: Bak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.
• Synonyms: BAK; BAK-LIKE; BCL2L7; CDN1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane