Amyloid Precursor Protein (APP) Rabbit Polyclonal Antibody

CAT#: TA326769

APP Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using APP antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1:2000;IHC: 1:50-1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: N term -peptide of human APP
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: amyloid beta precursor protein
• Database Link:
  NP_000475
  Entrez Gene 351 Human
  UniProt ID P05067
• Background: Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDatransmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (Aβ), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle-dependent and peaks during G2/M-phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.
• Synonyms: AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease