SIRT2 Rabbit Polyclonal Antibody

CAT#: TA326812

Rabbit anti-SIRT2 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using SIRT2 antibody.

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  Immunofluorescence analysis of HeLa cell using SIRT2 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:10 - 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human SIRT2
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: sirtuin 2
• Database Link:
  NP_036369
  Entrez Gene 64383 MouseEntrez Gene 361532 RatEntrez Gene 22933 Human
  UniProt ID Q8IXJ6
• Background: Sirtuins are members of the NAD-dependent histone deacetylase family of proteins that participate in a variety of cellular functions, including histone deacetylation, gene silencing, chromosomal stability, and aging. SIRT2, a human homolog of the yeast SIR2 (silent information regulator-2), functions as transcriptional silencing mediator at mating-type loci, telomeres and ribosomal gene clusters. SIRT2 expression increases dramatically during mitosis and is multiply phosphorylated at the G(2)/M transition of the cell cycle. SIRT2 is part of a phosphorylation cascade where it is phosphorylated late in G(2), during M, and into the period of cytokinesis. Inhibition of SIRT2 is reported to rescue alpha-synuclein toxicity and modify inclusion morphology in a cellular model of Parkinsons disease.
• Synonyms: SIR2; SIR2L; SIR2L2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors