N Cadherin (CDH2) Rabbit Polyclonal Antibody

CAT#: TA326835

N-cadherin Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts from C6 cell lysate using N-cadherin antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000-1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human N-cadherin
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cadherin 2
• Database Link:
  NP_001783
  Entrez Gene 12558 MouseEntrez Gene 83501 RatEntrez Gene 1000 Human
  UniProt ID P19022
• Background: Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, ?-catenin (also called plakoglobin), and p120 catenin. β-catenin and ?-catenin associate with a-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and ?-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers. Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers.
• Synonyms: CD325; CDHN; CDw325; NCAD

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs)