Sodium Potassium ATPase (ATP1A1) Rabbit Polyclonal Antibody

CAT#: TA326844

Rabbit polyclonal anti-ATP1A1(NaK ATPase) antibody, Loading control

Product Images

Western blot analysis of extracts of A549 cell and 293 cell line, using ATP1A1 antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:500 - 1:1000;IHC 1:20 - 1:200;IP 1:20 - 1:50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human ATP1A1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 113 kDa
• Gene Name: ATPase Na+/K+ transporting subunit alpha 1
• Database Link:
  NP_000692
  Entrez Gene 11928 MouseEntrez Gene 24211 RatEntrez Gene 476 Human
  UniProt ID P05023
• Background: The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic a subunit and a β subunit . Several phosphorylation sites have been identified for the a1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase , Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA . All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule . Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the a subunit . Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors.
• Synonyms: MGC3285; MGC51750

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction