Caspase 1 (CASP1) Rabbit Polyclonal Antibody

CAT#: TA326866

CASP1 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CASP1 antibody.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human CASP1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: caspase 1
• Database Link:
  NP_001214
  Entrez Gene 12362 MouseEntrez Gene 25166 RatEntrez Gene 834 Human
  UniProt ID P29466
• Background: Caspase-1, or interleukin-1β converting enzyme (ICE/ICEa), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1β and interferon-? inducing factor (IL-18) into their mature forms . Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis . Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock . At least six caspase-1 isoforms have been identified, including caspase-1 a, β, ?, d, e and ? . Most caspase-1 isoforms (a, β, ? and d) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 e typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ? isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the a isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 .
• Synonyms: ICE; IL1BC; P45

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway