Synaptotagmin 1 (SYT1) Rabbit Polyclonal Antibody

CAT#: TA326884

SYT1 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of various cell lines, using SYT1 antibody.

• 

  Immunofluorescence analysis of HeLa cell using SYT1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human SYT1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: synaptotagmin 1
• Database Link:
  NP_005630
  Entrez Gene 20979 MouseEntrez Gene 25716 RatEntrez Gene 6857 Human
  UniProt ID P21579
• Background: Synaptotagmin 1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis. Individual SYT1 proteins are composed of an amino-terminal transmembrane region, a central linker region and a pair of carboxy-terminal C2 domains responsible for binding Ca2+. The C2 domains appear to be functionally distinct, with the C2A domain responsible for regulating synaptic vesicle fusion in a calcium-dependent manner during exocytosis while the C2B domain allows for interaction between adjacent SYT1 proteins. Because synaptotagmin 1 binds calcium and is found in synaptic vesicles, this integral membrane protein is thought act as a calcium sensor in fast synaptic vesicle exocytosis. Evidence suggests possible roles in vesicle-mediated endocytosis and glucose-induced insulin secretion as well. SYT1 binds several different SNARE proteins during calcium-mediated vesicle endocytosis and an association between SYT1 and the SNARE protein SNAP-25 is thought to be a key element in vesicle-mediated exocytosis.
• Synonyms: P65; SVP65; SYT

Reference Data

• Protein Families: Secreted Protein, Transmembrane