14-3-3 sigma (SFN) Rabbit Polyclonal Antibody

CAT#: TA326895

Rabbit anti-SFN Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using SFN antibody.

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  Immunohistochemistry of paraffin-embedded mouse kidney using SFN antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat kidney using SFN antibody at dilution of 1:100 (400x lens).

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  Immunofluorescence analysis of MCF7 cell using SFN antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000, IHC 1:50- 1:200, IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human SFN
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: stratifin
• Database Link:
  NP_006133
  Entrez Gene 55948 MouseEntrez Gene 313017 RatEntrez Gene 2810 Human
  UniProt ID P31947
• Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.
• Synonyms: YWHAS

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cell cycle, p53 signaling pathway