APE1 (APEX1) Rabbit Polyclonal Antibody

CAT#: TA326935

Rabbit anti-APEX1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of Hela cell and HepG2 cell, using APEX1 antibody.

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  Immunofluorescence analysis of HeLa cell using APEX1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50- 1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human APEX1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: apurinic/apyrimidinic endodeoxyribonuclease 1
• Database Link:
  NP_001632
  Entrez Gene 328 Human
  UniProt ID P27695
• Background: Ape1 (Apurinic/Apyrimidic eEndonuclease 1), also known as Ref1 (Redox effector factor 1), is a multifunctional protein with several biological activities. These include roles in DNA repair and in the cellular response to oxidative stress. Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway. Repair activities of Ape1 are stimulated by interaction with XRCC1, another essential protein in BER. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation. Increased expression of Ape1 is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors . Ape1 has been shown to stimulate DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-¦ÊB, PAX, HIF-1, HLF and p53 . Mutation of the Ape1 gene has also been associated with amyotrophic lateral sclerosis (ALS).
• Synonyms: APE; APE1; APEN; APEX; APX; HAP1; REF1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair