MCM7 Rabbit Polyclonal Antibody

CAT#: TA326944

Rabbit anti-MCM7 Polyclonal Antibody

Product Images

Western blot analysis of extracts of variouscell lines, using MCM7 antibody.

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  Immunohistochemistry of paraffin-embedded human breast cancer using MCM7 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded human esophageal cancer using MCM7 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded human rectal cancer using MCM7 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat brain using MCM7 antibody at dilution of 1:200 (400x lens).

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  Immunofluorescence analysis of HeLa cell using MCM7 antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000, IHC 1:50- 1:200, IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human MCM7
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: minichromosome maintenance complex component 7
• Database Link:
  NP_005907
  Entrez Gene 17220 MouseEntrez Gene 288532 RatEntrez Gene 4176 Human
  UniProt ID P33993
• Background: The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork. This complex is also a key component of the pre-replication complex (pre-RC). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication. Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis. MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest.
• Synonyms: CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle, DNA replication