alpha Actinin (ACTN1) Rabbit Polyclonal Antibody

CAT#: TA326960

Rabbit anti-ACTN1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines using ACTN1 antibody.

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  Immunohistochemistry of paraffin-embedded human kidney using ACTN1 antibody at dilution of 1:200 (200x lens).

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  Immunohistochemistry of paraffin-embedded human stomach cancer using ACTN1 antibody at dilution of 1:200 (400x lens).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human, Mouse, Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ACTN1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: actinin alpha 1
• Database Link:
  NP_001093
  Entrez Gene 109711 MouseEntrez Gene 87 Human
  UniProt ID P12814
• Background: a-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently,a-Actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting. The interaction of a-Actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth. In osteoblasts, interaction of a-Actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis. Isoforms 1 and 4 of a-Actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle.
• Synonyms: BDPLT15

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction