FEN1 Rabbit Polyclonal Antibody

CAT#: TA326972

Rabbit anti-FEN1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using FEN1 antibody.

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  Immunohistochemistry of paraffin-embedded human lung cancer using FEN1 antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded mouse liver using FEN1 antibody at dilution of 1:100 (400x lens).

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  Immunofluorescence analysis of A549 cell using FEN1 antibody.

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  Immunofluorescence analysis of HeLa cell using FEN1 antibody.

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  Immunofluorescence analysis of HeLa cell using FEN1 antibody.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:10 - 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human FEN1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: flap structure-specific endonuclease 1
• Database Link:
  NP_004102
  Entrez Gene 14156 MouseEntrez Gene 84490 RatEntrez Gene 2237 Human
  UniProt ID P39748
• Background: Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways. The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers. FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair. The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions, post-translational modifications, and subcellular re-localization in response to cell cycle or DNA damage.
• Synonyms: FEN-1; MF1; RAD2

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, DNA replication, Non-homologous end-joining