PDGF beta (PDGFB) Rabbit Polyclonal Antibody

CAT#: TA326987

Rabbit anti-PDGFB Polyclonal Antibody

Product Images

Western blot analysis of extracts of A431 cell lines using PDGFB antibody.

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  Immunohistochemistry of paraffin-embedded human stomach cancer using PDGFB antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat spleen using PDGFB antibody at dilution of 1:200 (400x lens).

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PDGFB
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: platelet derived growth factor subunit B
• Database Link:
  NP_002599
  Entrez Gene 24628 RatEntrez Gene 5155 Human
  UniProt ID P01127
• Background: PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis. PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells. PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB. PDGF-BB binds to PDGFR¦Â and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLC¦Ã pathways. PDGF-BB may have a role in some cancer types.
• Synonyms: c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma