UPF1 Rabbit Polyclonal Antibody

CAT#: TA327064

UPF1 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using UPF1 antibody.

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  Immunofluorescence analysis of HeLa cell using UPF1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:200
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124kDa
• Gene Name: UPF1, RNA helicase and ATPase
• Database Link:
  NP_002902
  Entrez Gene 19704 MouseEntrez Gene 684558 RatEntrez Gene 5976 Human
  UniProt ID Q92900
• Background: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.
• Synonyms: HUPF1; NORF1; pNORF1; RENT1; smg-2

Reference Data

• Protein Families: Druggable Genome