UPF1 Rabbit Polyclonal Antibody

CAT#: TA327064

UPF1 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using UPF1 antibody.

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  Immunofluorescence analysis of HeLa cell using UPF1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human UPF1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: UPF1, RNA helicase and ATPase
• Database Link:
  NP_002902
  Entrez Gene 19704 MouseEntrez Gene 684558 RatEntrez Gene 5976 Human
  UniProt ID Q92900
• Background: Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm and was later shown to be a component of cytoplasmic P-bodies . Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery. Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively.
• Synonyms: HUPF1; NORF1; pNORF1; RENT1; smg-2

Reference Data

• Protein Families: Druggable Genome