CDC25C Rabbit Polyclonal Antibody

CAT#: TA327133

CDC25C Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CDC25C antibody.

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  Immunohistochemistry of paraffin-embedded rat brain using CDC25C antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat kidney using CDC25C antibody at dilution of 1:100 (400x lens).

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  Immunofluorescence analysis of U2OS cell using CDC25C antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000, IHC 1:50- 1:200, ICC 1:50- 1:200, IP 1:50- 1:100, IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human CDC25C
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cell division cycle 25C
• Database Link:
  NP_001781
  Entrez Gene 12532 MouseEntrez Gene 307511 RatEntrez Gene 995 Human
  UniProt ID P30307
• Background: Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis. cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint. When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis. The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage.
• Synonyms: CDC25; PPP1R60

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation