PAX3 Rabbit Polyclonal Antibody

CAT#: TA327135

PAX3 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of A431 cell lines, using PAX3 antibody.

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  Immunohistochemistry of paraffin-embedded human lung cancer using PAX3 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat liver using PAX3 antibody at dilution of 1:200 (400x lens). I

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  Immunohistochemistry of paraffin-embedded rat lung using PAX3 antibody at dilution of 1:200 (400x lens).

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  Immunofluorescence analysis of MCF7 cell using PAX3 antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PAX3
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: paired box 3
• Database Link:
  NP_852122
  Entrez Gene 18505 MouseEntrez Gene 114502 RatEntrez Gene 5077 Human
  UniProt ID P23760
• Background: Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region. PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis; a subset of PAX proteins also maintain functional importance during postnatal development. Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes, with members of subgroups II and III identified as potential mediators of tumor progression.
• Synonyms: CDHS; HUP2; WS1; WS3

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors