PSAP Rabbit Polyclonal Antibody

CAT#: TA327188

Rabbit anti-PSAP Polyclonal Antibody

Product Images

Western blot analysis of extracts of HEK-293 cell lines, using PSAP antibody.

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  Immunofluorescence analysis of MCF7 cell using PSAP antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PSAP
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: prosaposin
• Database Link:
  NP_002769
  Entrez Gene 19156 MouseEntrez Gene 25524 RatEntrez Gene 5660 Human
  UniProt ID P07602
• Background: The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed sphingolipid activator proteins (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. They are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases.Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.
• Synonyms: GLBA; SAP1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysosome