TRIM21 Rabbit Polyclonal Antibody

CAT#: TA327221

Rabbit anti-TRIM21 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TRIM21 antibody.

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  Immunohistochemistry of paraffin-embedded mouse heart using TRIM21 antibody at dilution of 1:100 (x40 lens).

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  Immunofluorescence analysis of U2OS cell using TRIM21 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:10 - 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human TRIM21
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: tripartite motif containing 21
• Database Link:
  NP_003132
  Entrez Gene 20821 MouseEntrez Gene 308901 RatEntrez Gene 6737 Human
  UniProt ID P19474
• Background: TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules.
• Synonyms: RNF81; Ro; RO52; SSA; SSA1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus