OGT Rabbit Polyclonal Antibody

CAT#: TA327225

OGT Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using OGT antibody.

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  Immunohistochemistry of paraffin-embedded rat heart using OGT antibody at dilution of 1:100 (x40 lens).

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  Immunohistochemistry of paraffin-embedded human liver injury using OGT antibody at dilution of 1:100 (x40 lens).

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  Immunohistochemistry of paraffin-embedded rat lung using OGT antibody at dilution of 1:100 (400x lens).

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  Immunofluorescence analysis of U2OS cell using OGT antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human OGT
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 117 kDa
• Gene Name: O-linked N-acetylglucosamine (GlcNAc) transferase
• Database Link:
  NP_858058
  Entrez Gene 26295 RatEntrez Gene 108155 MouseEntrez Gene 8473 Human
  UniProt ID O15294
• Background: This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Synonyms: HRNT1; O-GLCNAC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis