CD73 (NT5E) Rabbit Polyclonal Antibody

CAT#: TA327230

NT5E Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using NT5E antibody.

• 

  Immunohistochemistry of paraffin-embedded mouse lung using NT5E antibody at dilution of 1:100 (400x lens).

• 

  Immunofluorescence analysis of MCF7 cell using NT5E antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human NT5E
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: 5'-nucleotidase ecto
• Database Link:
  NP_002517
  Entrez Gene 23959 MouseEntrez Gene 58813 RatEntrez Gene 4907 Human
  UniProt ID P21589
• Background: Ecto-5nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleoside. NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine-monophosphate, therefore driving the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors. Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promotor leads to upregulation of NT5E during hypoxia. NT5E biological roles span from lymphocyte adhesion, to fibrosis, to regulation of nociception.
• Synonyms: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism