ERK5 (MAPK7) Rabbit Polyclonal Antibody

CAT#: TA327276

MAPK7 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MAPK7 antibody.

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  Immunohistochemistry of paraffin-embedded human esophageal cancer using MAPK7 antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded human esophagus using MAPK7 antibody at dilution of 1:100 (400x lens).

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human MAPK7
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mitogen-activated protein kinase 7
• Database Link:
  NP_002740
  Entrez Gene 23939 MouseEntrez Gene 114509 RatEntrez Gene 5598 Human
  UniProt ID Q13164
• Background: ERK5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival. Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism. For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets. ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where it promotes survival and proliferation. In neuronal cells, ERK5 is required for NGF-induced neurite outgrowth, neuronal homeostatis, and survival. ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function. Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis.
• Synonyms: BMK1; ERK4; ERK5; PRKM7

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway