TNFAIP3 Rabbit Polyclonal Antibody

CAT#: TA327286

TNFAIP3 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various mouse tissues, using TNFAIP3 antibody

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  Immunohistochemistry of paraffin-embedded human stomach cancer using TNFAIP3 antibody at dilution of 1:200 (400x lens).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human TNFAIP3
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: TNF alpha induced protein 3
• Database Link:
  NP_006281
  Entrez Gene 21929 MouseEntrez Gene 683206 RatEntrez Gene 7128 Human
  UniProt ID P21580
• Background: A20, also referred to as TNF-a-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-?B. It was first identified as a TNF-a inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs. Constitutive expression of A20 is observed in lymphoid tissues, but it is transiently expressed in a variety of cell types in response to inflammatory signals such as TNF-a, IL-1, phorbol esters, and LPS . Expression of A20 can confer resistance to apoptosis and NF-?B activation triggered by these signals, probably through interference with TRAF (TNF receptor associated factor) family members , and interaction with the NF-?B inhibiting protein ABIN. Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions. The amino-terminus of A20 contains de-ubiquitinating (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation .
• Synonyms: A20; OTUD7C; TNFA1P2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway