ATF2 Rabbit Polyclonal Antibody

CAT#: TA327301

ATF2 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cells, using ATF2 antibody.

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  Immunohistochemistry of paraffin-embedded human normal breast using ATF2 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded human breast cancer using ATF2 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded human normal thyroid using ATF2 antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded human stomach cancer using ATF2 antibody at dilution of 1:200 (400x lens).

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ATF2
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56 kDa
• Gene Name: activating transcription factor 2
• Database Link:
  NP_001871
  Entrez Gene 11909 MouseEntrez Gene 81647 RatEntrez Gene 1386 Human
  UniProt ID P15336
• Background: The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins . ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways . Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 . Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 . In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 .
• Synonyms: CRE-BP1; CREB-2; CREB2; HB16; TREB7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway