Isocitrate dehydrogenase (IDH1) Rabbit Polyclonal Antibody

CAT#: TA327310

Rabbit anti-IDH1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of MCF7 cell lines, using IDH1 antibody.

• 

  Immunofluorescence analysis of HeLa cell using IDH1 antibody.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat, Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human IDH1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: isocitrate dehydrogenase (NADP(+)) 1, cytosolic
• Database Link:
  NP_005887
  Entrez Gene 15926 MouseEntrez Gene 24479 RatEntrez Gene 710019 MonkeyEntrez Gene 3417 Human
  UniProt ID O75874
• Background: IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to a-ketoglutarate (a-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor. IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock. IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
• Synonyms: HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways