H3FT (HIST3H3) Rabbit Polyclonal Antibody

CAT#: TA327348

MonoMethyl-Histone H3-K27 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K27me1 antibody.

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  Immunohistochemistry of paraffin-embedded rat brain tissue using H3K27me1 antibody at dilution of 1:200 (x400 lens).

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  Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using H3K27me1 antibody at dilution of 1:200 (x400 lens).

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  Immunohistochemistry of paraffin-embedded rat lung tissue using H3K27me1 antibody at dilution of 1:200 (x400 lens).

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  Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K27me1 antibody at dilution of 1:200 (x400 lens).

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  Immunofluorescence analysis of 293T cell using H3K27me1 antibody. Blue: DAPI for nuclear staining.

• Dot-blot analysis of all sorts of methylation peptides using H3K27me1 antibody.

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Specifications

Product Data

• Applications: ChIP, Dot, ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:50 - 1:200;ChIP 1:20 - 1:100
• Reactivities: Human, Mouse, Rat, Other (Wide Range)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15 kDa
• Gene Name: histone cluster 3, H3
• Database Link:
  NP_003484
  Entrez Gene 691496 RatEntrez Gene 8290 Human
  UniProt ID Q16695
• Background: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
• Synonyms: g; H3; H3.4; H3FT; H3t

Reference Data

• Protein Pathways: Systemic lupus erythematosus