CXCR3 Rabbit Polyclonal Antibody

CAT#: TA327450

Rabbit anti-CXCR3 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CXCR3 antibody (TA327450) at 1:400 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Western blot analysis of extracts of various cell lines, using CXCR3 antibody (TA327450) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunohistochemistry of paraffin-embedded human liver cancer using CXCR3 Antibody (TA327450) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse skeletal muscle using CXCR3 Antibody (TA327450) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse heart using CXCR3 Antibody (TA327450) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1:2000;IHC: 1:50-1:100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human CXCR3
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: C-X-C motif chemokine receptor 3
• Database Link:
  NP_001495
  Entrez Gene 12766 MouseEntrez Gene 2833 Human
  UniProt ID P49682
• Background: This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats.
• Synonyms: CD182; CD183; CKR-L2; CMKAR3; GPR9; IP10-R; Mig-R; MigR

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction