RRM2 Rabbit Polyclonal Antibody

CAT#: TA327571

RRM2 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using RRM2 antibody.

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  Immunohistochemistry of paraffin-embedded rat heart using RRM2 antibody at dilution of 1:100 (x400 lens).

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  Immunohistochemical of paraffin-embedded human kidney using RRM2 antibody at dilution of 1:200 (200x lens).

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  Immunofluorescence analysis of HeLa cell using RRM2 antibody. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of A549 cell using RRM2 antibody. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:10 - 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human RRM2
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: ribonucleotide reductase regulatory subunit M2
• Database Link:
  NP_001025
  Entrez Gene 20135 MouseEntrez Gene 362720 RatEntrez Gene 6241 Human
  UniProt ID P31350
• Background: Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5-diphosphates into 2-deoxyribonucleotides, a rate-limiting step in the production of 2-deoxyribonucleoside 5-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit.
• Synonyms: R2; RR2; RR2M

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways, p53 signaling pathway, Purine metabolism, Pyrimidine metabolism