PAX8 Mouse Monoclonal Antibody [Clone ID: MRQ-50]

CAT#: TA327724

Mouse Monoclonal Pax-8 Antibody

Product Images

Immunohistochemistry staining of Paraffin Ovarian carcinoma (non-mucinous carcinoma) tissue by Pax-8 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear)

Specifications

Product Data

• Clone Name: MRQ-50
• Applications: IHC
• Recommended Dilution: IHC: 1:50 - 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Formulation: This antibody is supplied as cell culture supernatant diluted in tris buffered saline, pH 7.3-7.7, with 1% BSA and <0.1% sodium azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: paired box 8
• Database Link:
  NP_003457
  Entrez Gene 7849 Human
  UniProt ID Q06710
• Synonyms: OTTHUMP00000158659; OTTHUMP00000158660; paired box 8; paired domain gene 8
• Note: This protein is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins which contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical thyroid adenomas.PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. Studies have also found PAX-8 expression in renal tubules as well as renal carcinoma, nephroblastoma, and seminoma. Studies have shown that 98% of clear cell RCCs, 90% of papillary RCCs, and 95% of oncocytomas are positive for anti-PAX-8, frequencies which are similar to or better than those for anti-PAX-2. Therefore, anti-PAX-8 can be used as an additional immunohistochemical marker for renal epithelial tumors. Normal lung and lung carcinomas do not express PAX-8. Anti-PAX-8, combined with organ system-specific markers such as anti-uroplakin, anti-mammaglobin, and anti-TTF-1 can be a very useful panel to determine the primary site of invasive micropapillary carcinomas from ovary (positive staining) or from bladder, lung, and breast (negative staining). Anti-PAX-8 is also useful in distinguishing ovarian serous carcinoma from malignant mesothelioma of peritoneum.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Thyroid cancer