Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: V9]

CAT#: TA327753

Mouse Monoclonal Vimentin Antibody

Product Images

Immunohistochemistry staining of Paraffin Tonsil tissue by Vimentin antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)

Specifications

Product Data

• Clone Name: V9
• Applications: IHC
• Recommended Dilution: IHC: 1:100 - 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Formulation: The antibody is diluted in tris buffered saline, pH 7.3-7.7, with 1% BSA and <1% sodium azide.
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: vimentin
• Database Link:
  NP_003371
  Entrez Gene 7431 Human
  UniProt ID P08670
• Synonyms: CTRCT30; HEL113
• Note: Anti-vimentin is of limited value as a diagnostic tool; however, when used in combination with other antibodies (in panels) it is useful for the subclassification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. This antibody recognizes a 57 kD intermediate filament. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Reference Data

• Protein Families: ES Cell Differentiation/IPS