p53 (TP53) Rabbit Polyclonal Antibody [Clone ID: Poly6142]

CAT#: TA327919

Rabbit Polyclonal anti-p53-Acetylated (Lys382) Antibody

Product Images

MCF-7 cells were treated with 50 uM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells.

Specifications

Product Data

• Clone Name: Poly6142
• Applications: WB
• Recommended Dilution: WB
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Modified peptide
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
• Purification: The antibody was purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53 kD
• Gene Name: tumor protein p53
• Database Link:
  NP_000537
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73. The Poly6142 antibody recognizes human acetylated p53 (Lys382) and has been shown to be useful for Western blotting.
• Synonyms: BCC7; LFS1; P53; TRP53
• Note: Reacts against Lys382-acetylated p53

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway