PARP1 Mouse Monoclonal Antibody [Clone ID: 5A5]

CAT#: TA327935

Mouse Monoclonal anti-PARP Antibody

Product Images

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PARP antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.

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  Hela cells were stained with PE anti-PARP antibody and DAPI. The image shows nuclear localization of PARP.

Specifications

Product Data

• Clone Name: 5A5
• Applications: IF, WB
• Recommended Dilution: IF, WB
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Recombinant (partial), N-terminal 2/3 sequence of PARP
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 113 kD
• Gene Name: poly(ADP-ribose) polymerase 1
• Database Link:
  NP_001609
  Entrez Gene 11545 MouseEntrez Gene 142 Human
  UniProt ID P09874
• Background: PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.
• Synonyms: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair