KAT2B Mouse Monoclonal Antibody [Clone ID: 15G10]

CAT#: TA327982

Mouse Monoclonal anti-Acetylated Lysine Antibody

Product Images

Untreated Hela cells (lane 1) and sodium butyrate-treated Hela cells (24 hr treatment, lane 2) were lysed and cell extracts resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetylated lysine antibody (clone 15G10). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.

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  Untreated Hela cells (Upper Panel), or overnight nocodazole treated Hela cells (Lower Panel) stained with purified mouse monoclonal antibody against Acetylated Lysine (clone 15G10), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.

Specifications

Product Data

• Clone Name: 15G10
• Applications: IF, WB
• Recommended Dilution: WB, IF
• Reactivities: Acetylated lysine residues
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: Acetylated protein mixture
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lysine acetyltransferase 2B
• Database Link:
  NP_003875
  Entrez Gene 8850 Human
  UniProt ID Q92831
• Background: Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetytransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.
• Synonyms: CAF; P; PCAF

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway