KAP1 (TRIM28) Mouse Monoclonal Antibody [Clone ID: 20A1]

CAT#: TA328012

Mouse Monoclonal anti-TIF1β Antibody

Product Images

HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1ß antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

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  HeLa cells were intracellularly stained with 5 ug/ml of purified anti-TIF1β (KAP-1, TRIM28) (clone 20A1) in blocking buffer overnight at 4?, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.

Specifications

Product Data

• Clone Name: 20A1
• Applications: IF, WB
• Recommended Dilution: WB, IF, ChIP
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Not provided
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 110 kD
• Gene Name: tripartite motif containing 28
• Database Link:
  NP_005753
  Entrez Gene 10155 Human
  UniProt ID Q13263
• Background: TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits setdbl to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with setdbl and cbx3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
• Synonyms: KAP1; PPP1R157; RNF96; TF1B; TIF1B

Reference Data

• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors