Lamin A (LMNA) Rabbit Polyclonal Antibody [Clone ID: Poly6135]

CAT#: TA328054

Rabbit Polyclonal anti-Lamin A Antibody

Product Images

Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-lamin A. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

Specifications

Product Data

• Clone Name: Poly6135
• Applications: WB
• Recommended Dilution: IF, WB
• Reactivities: Human, weakly cross-reactive with Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant (partial), C-terminal
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
• Purification: The antibody was purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70 kD, 66 kD, and 61 kD
• Gene Name: lamin A/C
• Database Link:
  NP_733821
  Entrez Gene 16905 MouseEntrez Gene 4000 Human
  UniProt ID P02545
• Background: Lamin A is a member of the intermediate filament family that contains a farnesyl binding domain and forms dimers. Three isoforms of Lamin A have been reported designated A, AD10, C, with molecular weights of approximately 70 kD, 66 kD, and 61 kD, respectively. Lamin A is localized to the nucleoplasmic side of inner nuclear membrane. Lamin A is thought to function as a fibrous component of the nuclear lamina, providing a framework for the nuclear envelope, and possibly interacting with chromatin. Expression of lamin A is strictly under cell cycle control as seen by disintegration/formation of nuclear envelope in prophase/telophase. Lamin A is modified by phosphorylation, methylation, and farnesylation; phosphorylation regulates disassembly. Lamin A forms a homodimer with Lamin C and has also been shown to interact with the LAPs 1A-1C, emerin, Narf, hsMOK2, PKC, SREBP1a, and SREBP 1c. The Poly6135 antibody recognizes the C-terminal region of human and mouse Lamin A and has been shown to be useful for Western blotting and immunofluorescence staining.
• Synonyms: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)