Actb Rabbit Polyclonal Antibody [Clone ID: Poly6221]

CAT#: TA328070

Rabbit Polyclonal anti-β-Actin Antibody

Product Images

Western blot analysis of Hela (lane 1), mouse spleen (lane 2) and NIH3T3 (lane 3) cells, using anti-β-actin antibody (poly6221).

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  Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6221 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.

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  Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.

Specifications

Product Data

• Clone Name: Poly6221
• Applications: IF, IHC, WB
• Recommended Dilution: WB, IF, IHC
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide-KLH, NH2 terminus
• Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 KD
• Gene Name: actin, beta
• Database Link:
  NP_031419
  Entrez Gene 60 HumanEntrez Gene 81822 RatEntrez Gene 11461 Mouse
  UniProt ID P60710
• Background: β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin.
• Synonyms: Beta-actin; PS1TP5BP1