Prkdc Mouse Monoclonal Antibody [Clone ID: 10B1]

CAT#: TA328095

Mouse Monoclonal anti-DNA-PKcs Phospho (Thr2609) Antibody

Product Images

MOLT4 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-DNA-PKcs (Thr2609) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Lane 1, untreated MOLT4 cells. Lane 2, MOLT4 cells exposed to 2 Gy radiation. Lane 3, MOLT4 cells exposed to 10 Gy radiation. In both cases, cells were harvested 30 minutes after radiation.

Specifications

Product Data

• Clone Name: 10B1
• Applications: WB
• Recommended Dilution: WB, IF
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Modified peptide
• Formulation: This DNA-PKcs antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
• Concentration: lot specific
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 470 kD
• Gene Name: protein kinase, DNA activated, catalytic polypeptide
• Database Link:
  NP_035289
  Entrez Gene 5591 Human
  UniProt ID P97313
• Background: DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.
• Synonyms: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; p350; p460; XRCC7
• Note: reacts with Thr2609-phosphorylated DNA-PKcs