Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: 1291CT875.142.166]

CAT#: TA328117

Mouse monoclonal anti-TOP1 antibody (N-term)

Product Images

Western blot analysis of lysates from HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right), using TOP1 Antibody (N-term) (Cat. #TA328117). TA328117 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Specifications

Product Data

• Clone Name: 1291CT875.142.166
• Applications: WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
• Formulation: PBS with 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: topoisomerase (DNA) I
• Database Link:
  NP_003277
  Entrez Gene 7150 Human
  UniProt ID P11387
• Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
• Synonyms: TOPI

Reference Data

• Protein Families: Druggable Genome