Her2 (ERBB2) Rabbit Polyclonal Antibody

CAT#: TA328148

Rabbit polyclonal anti-Phospho-ErbB2(Y1221) antibody

Product Images

Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using phospho ErbB2-Y1221 (left) or ErbB2 antibody (right)

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  Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using phospho ErbB2-Y1221 (left) or ErbB2 antibody (right)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1221 of human ERBB2.
• Formulation: PBS with 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  NP_004439
  Entrez Gene 2064 Human
  UniProt ID P04626
• Background: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus it is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
• Synonyms: CD340; HER-2; HER2; MLN 19; NEU; neu; NGL; TKR1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer