IL10 Rabbit Polyclonal Antibody

CAT#: TA328225

Anti-Human IL-10 Rabbit Polyclonal Antibody

Product Images

To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (TA328224) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10.

• 

  To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (TA328224) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10.

• 

  To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 (TA328224) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Human IL-10
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-10. Anti-Human IL-10 specific antibody was purified by affinity chromatography employing immobilized hIL-10 matrix.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 10
• Database Link:
  NP_000563
  Entrez Gene 3586 Human
  UniProt ID P22301
• Synonyms: CSIF; GVHDS; IL-10; IL10A; TGIF
• Note: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-10 (1.0 ng/ml), a concentration of 1.2-1.8 <=ug/ml of this antibody is required.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway