BDNF Rabbit Polyclonal Antibody

CAT#: TA328268

Anti-Human BDNF Rabbit Polyclonal Antibody

Product Images

This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2.0 ug/ml overnight at 4C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Human BDNF
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF. Anti-Human BDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: brain-derived neurotrophic factor
• Database Link:
  NP_001700
  Entrez Gene 627 Human
  UniProt ID P23560
• Synonyms: ANON2; BULN2
• Note: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBDNF (2.0 ug/ml), a concentration of 0.54-0.81 ug/ml of this antibody is required.

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane
• Protein Pathways: Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway