EGF Rabbit Polyclonal Antibody

CAT#: TA328292

Anti-Human EGF Rabbit Polyclonal Antibody

Product Images

To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.

• 

  To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.

• 

  To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.

• 

  To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived recombinant Human EGF
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF. Anti-Human EGF specific antibody was purified by affinity chromatography employing immobilized hEGF matrix.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: epidermal growth factor
• Database Link:
  NP_001954
  Entrez Gene 1950 Human
  UniProt ID P01133
• Synonyms: HOMG4; URG
• Note: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (25.0 pg/ml), a concentration of < 0.1 <=ug/ml of this antibody is required.

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton