RANKL (TNFSF11) Rabbit Polyclonal Antibody

CAT#: TA328429

Anti-Human sRANK Ligand Rabbit Polyclonal Antibody

Product Images

To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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  This antibody stained formalin-fixed, paraffin-embedded sections of human metastatic carcinoma of lymph node from breast tissue. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Human sRANK Ligand
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsRANKL. Anti-Human sRANKL specific antibody was purified by affinity chromatography employing immobilized hsRANKL matrix.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor superfamily member 11
• Database Link:
  NP_003692
  Entrez Gene 8600 Human
  UniProt ID O14788
• Synonyms: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TRANCE

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction