TNF alpha (TNF) Goat Polyclonal Antibody

CAT#: TA328454

Anti-Human TNF-a Goat Polyclonal Antibody

Product Images

To detect Human TNF-alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha; (TA328453) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha;.

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  To detect Human TNF-alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha; (TA328453) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha;.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Human TNF-α
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-alpha. Anti-Human TNF-alpha specific antibody was purified by affinity chromatography employing immobilized Human TNF-alpha matrix.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor
• Database Link:
  NP_000585
  Entrez Gene 7124 Human
  UniProt ID P01375
• Synonyms: DIF; TNF-alpha; TNFA; TNFSF2
• Note: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.25 ng/ml), a concentration of 0.012 - 0.014 <=ug/ml of this antibody is required.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus