VEGFA Rabbit Polyclonal Antibody

CAT#: TA328471

Anti-Human VEGF Rabbit Polyclonal Antibody

Product Images

This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 2.5-5.5mg/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Human VEGF
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVEGF (human VEGF). Anti-Human VEGF specific antibody was purified by affinity chromatography employing immobilized hVEGF matrix.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: vascular endothelial growth factor A
• Database Link:
  NP_001028928
  Entrez Gene 7422 Human
  UniProt ID P15692
• Synonyms: MVCD1; VEGF; VPF
• Note: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hVEGF (10 ng/ml), a concentration of 0.05 - 0.1 <=ug/ml of this antibody is required.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway