Il1b Rabbit Polyclonal Antibody

CAT#: TA328479

Anti-Murine IL-1β Rabbit Polyclonal Antibody

Product Images

To detect mIL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1beta (TA328478) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta.

• 

  To detect mIL-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1beta (TA328478) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
• Reactivities: Murine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Murine IL-1β
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1beta. Anti-Murine IL-1beta specific antibody was purified by affinity chromatography employing immobilized mIL-1beta matrix.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 1 beta
• Database Link:
  NP_032387
  Entrez Gene 16176 Mouse
  UniProt ID P10749
• Synonyms: catabolin; IL-1; IL-1B; IL1-BETA; IL1F2; pro-interleukin-1-beta
• Note: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1beta (10 pg/ml), a concentration of 0.57-0.86 <=ug/ml of this antibody is required.