Tnf Rabbit Polyclonal Antibody

CAT#: TA328597

Anti-Rat TNF-a Rabbit Polyclonal Antibody

Product Images

To detect Rat TNF-alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat TNF-alpha; (TA328596) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-alpha;.

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: WB: 0.1-0.2ug/mL, ELISA: 0.25-2ug/mL, IHC: 0.25ug/mL - 1mg/mL
• Reactivities: Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E.coli derived Recombinant Rat TNF-α
• Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Purification: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat TNF-alpha. Anti-Rat TNF-alpha specific antibody was purified by affinity chromatography employing immobilized Rat TNF-alpha matrix.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor
• Database Link:
  NP_036807
  Entrez Gene 24835 Rat
• Synonyms: Cachectin; DIF; TNF-a; TNF-alpha; TNFA; TNFSF2
• Note: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF-alpha (2.0 ng/ml), a concentration of 0.07-0.10 <=ug/ml of this antibody is required.